SpyRho 555
SpyRho 555 是一种用于活细胞 RNA 成像的荧光激活染料配体。该染料具有细胞渗透性优异、荧光激活性强和光稳定性好的特点，可与罗丹明结合适体RhoBAST 配合实现活细胞 RNA 成像，适用于活细胞或固定细胞。
产品包含 1 瓶 SpyRho 555（可满足 100 次染色实验需求）。
产品描述
SpyRho 555 是由海德堡大学 Murat Sunbul 课题组开发的 RNA 适体 RhoBAST 的荧光激活染料配体1)。该罗丹明染料具有细胞渗透性优异、荧光激活性强和光稳定性好的特点。凭借其与 RhoBAST 适体的高亲和力及快速结合/解离动力学特性，可有效解决活细胞 RNA 成像的主要难题。SpyRho 555 特别适用于宽场、共聚焦、STED 或类似 PAINT 技术的单分子定位显微成像。
SpyRho 555 可与 SPY505、SPY595、SPY650、SPY700、SiR 及 GFP 进行多色成像，使用标准TMR 或 Cy3 参数设置即可成像。*每份探针可满足 100 次染色实验需求。
Figure 1. A Chemical structure of SpyRho 555. B SpyRho 555 reversibly binds to RhoBAST aptamer with fast kinetics. Unbound SpyRho 555 exists predominantly in the closed form and is not fluorescent. Upon binding to RhoBAST aptamer, SpyRho 555 switches to the open form and becomes highly fluorescent.
产品参数
| Absorbance maximum λabs | 562 nm |
| Fluorescence maximum λfl | 581 nm |
| Works on fixed cells? | Yes |
| Probe quantity | 100 stainings* |
| Fluorescence quantum yield (RNA bound) | 0.95 |
| Kd to RhoBAST aptamer | 34 nM |
| STED depletion wavelength | 660 nm |
| MW | 492.5 g/mol |
| Shipping | room temperature |
| Storage | -20°C |
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
1. Daniel Englert et al. Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging” bioRxiv 2022.10.24.513449 https://doi.org/10.1101/2022.10.24.513449
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